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Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore
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Flavin-tag: we
developed a facile method to label a target protein with a flavin. By using a
short peptide (Flavin-tag), recognized by a flavinylating enzyme, FMN can be
covalently incorporated. Attachment of the flavin chromo- and fluorophore can
be performed
in vivo
and
in vitro
rendering the Flavin-tag a
flexible tool for protein labeling.
Title: Flavin-Tag: A Facile Method for Site-Specific Labeling of Proteins with a Flavin Fluorophore
Description:
Flavin-tag: we
developed a facile method to label a target protein with a flavin.
By using a
short peptide (Flavin-tag), recognized by a flavinylating enzyme, FMN can be
covalently incorporated.
Attachment of the flavin chromo- and fluorophore can
be performed
in vivo
and
in vitro
rendering the Flavin-tag a
flexible tool for protein labeling.
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