Tissue culture and antimicrobial activity of crude extract from leaf and stem of might blooming jasmine Nyctanthes arbor-tristis L.

Tissue of Nyctanthes arbor-tristis L. was cultured in vitro for multiplication shown successive shoot induction from intemode cultured in MS medium containing 0.4 mg/1 BA. Multiple shoot could be obtained culture from intemode segments on MS medium supplemented with 0.4 mg/1 BA and 0.1 mg/1 NAA. Supplementation of 2,4-D at 0.1 mg/1 in MS medium without non activated charcoal could induce root formation. Crude extracts from leaf and stem of mother plants from Nyctanthes arbor-tristis L. were shown to inhibit growth of E. coli and B. substilis at 0.15 mg/ml, 0.075 ing/ml while antifimgus activity in ร. cerevisiea and Aspergillus sp. were shown at 0.15 mg/ml only. The extracts of leaves and stems from tissue culture showed microbial activity in E. coli, B. substilis and ร. cerevisiea at 0.15 mg/ml while in Aspergillus sp. were not found. After that, Crude extracts of Nyctanthes arbor-tristis L. from mother plants and tissue culture (leaves and stems) showed inhibition of growth of bacteria: E. coli, B. substilis while they showed partial inhibition of growth in S. cerevisiea and Aspergillus sp. Extraction of carotenoid from corolla tube of Nyctanthes arbor-tristis L. were detected by TLC with Rf = 0.93.