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Isotopic labeling of freshwater mixotrophic algae using isotopic labeled heat-killed bacteria v1
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This protocol aims to achieve an isotopic labeling of mixotrophic algae using 15Nand 13Clabeled heat-killed bacteria. It was developped to be used with the mixorophic chrysophyteOchromonas to asses the sources of carbon and nitrogen of this algae when growing mixotrophically .Sources of nitrogen and carbon in the media used were reduced to 1 inorganic source (NH4Cl for nitrogen and bicarbonate for carbon) and 1 organic source (heat-killed bacteria); media used for Ochromonas growth was modified accordingly to reflect this. Downstream analysis of isotopic labeled Ochromonas includebulk IRMS measurements or nanoSIMS measurements.
Title: Isotopic labeling of freshwater mixotrophic algae using isotopic labeled heat-killed bacteria v1
Description:
This protocol aims to achieve an isotopic labeling of mixotrophic algae using 15Nand 13Clabeled heat-killed bacteria.
It was developped to be used with the mixorophic chrysophyteOchromonas to asses the sources of carbon and nitrogen of this algae when growing mixotrophically .
Sources of nitrogen and carbon in the media used were reduced to 1 inorganic source (NH4Cl for nitrogen and bicarbonate for carbon) and 1 organic source (heat-killed bacteria); media used for Ochromonas growth was modified accordingly to reflect this.
Downstream analysis of isotopic labeled Ochromonas includebulk IRMS measurements or nanoSIMS measurements.
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