In Silico and Experimental Analyses of Long Non-Coding RNA TMPO-AS1 Expression in Gastric Cancer Tissues

Abstract Background A large body of evidence has illustrated the prominent roles of non-coding RNAs, particularly long non-coding RNAs (lncRNAs), in cancer progression and diagnosis. In this study, we considered in silico and experimental analyses of long non-coding TMPO-AS1 expression in adjacent nonmalignant tissues and cancerous tissues of the patients with gastric cancer. Methods In order to conduct the present study, 40 gastric tumor samples and 40 marginal noncancerous counterparts were collected. The TMPO-AS1 expression levels in patients’ samples were evaluated by qRT-PCR analysis. The Cancer Genome Atlas (TCGA) data were retrieved for TMPO-AS1 and analyzed via Gepia and TANRIC online tools. Student t-test, one-way ANOVA, and chi-square test were accomplished via SPSS software. Results The data demonstrated that TMPO-AS1 was overexpressed in cancerous tissues compared to nonmalignant adjacent ones (p: 0.0076). The TCGA data demonstrated that TMPO-AS1 was upregulated in GC tissues in comparison to nonmalignant adjacent ones (p = 0.001). Conclusion Altogether, in our study, we demonstrated lncRNA TMPO-AS1 could be considered as a biomarker in GC.However, further investigations are required to indicate the potential application of this lncRNA in diagnosis, prognosis and therapeutic target of GC.