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Vegetative compatibility of Fusarium oxysporum f.sp. dianthi from carnation in Israel
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Auxotrophic mutants were used to determine vegetative relatedness among isolates of
Fusarium oxysporum
f.sp.
dianthi (F.o.d.)
, the vascular wilt pathogen of carnation. At the first stage, different nitrate‐non‐utilizing
(nit)
mutants were produced from 11 isolates of
F.o.d.
collected in Israel. Complementation (heterokaryon) tests showed that all the isolates belonged to a single vegetative compatibility group (VCG), and two mutants were chosen as its testers. Additional isolates of
Fusarium
from carnation, collected during 1986‐88, were analysed for pathogenicity and vegetative compatibility with the testers. A total of 170
Fusarium
isolates, obtained from 42 cultivars at 40 sites, were tested. All the
nit
mutants of all the 132 pathogenic isolates formed heterokaryons with the testers, indicating that they belonged to the same VCG. None of the 38 non‐pathogenic isolates was vegetatively compatible with the testers. The
nit
mutants retained pathogenicity
to
carnation. The
F.o.d.
testers were not compatible with testers of five
other formae speciales
of
F. oxysporum.
Thus,
F.o.d.
appears to constitute a distinct genetic population within the
F. oxysporum
complex.
Title: Vegetative compatibility of Fusarium oxysporum f.sp. dianthi from carnation in Israel
Description:
Auxotrophic mutants were used to determine vegetative relatedness among isolates of
Fusarium oxysporum
f.
sp.
dianthi (F.
o.
d.
)
, the vascular wilt pathogen of carnation.
At the first stage, different nitrate‐non‐utilizing
(nit)
mutants were produced from 11 isolates of
F.
o.
d.
collected in Israel.
Complementation (heterokaryon) tests showed that all the isolates belonged to a single vegetative compatibility group (VCG), and two mutants were chosen as its testers.
Additional isolates of
Fusarium
from carnation, collected during 1986‐88, were analysed for pathogenicity and vegetative compatibility with the testers.
A total of 170
Fusarium
isolates, obtained from 42 cultivars at 40 sites, were tested.
All the
nit
mutants of all the 132 pathogenic isolates formed heterokaryons with the testers, indicating that they belonged to the same VCG.
None of the 38 non‐pathogenic isolates was vegetatively compatible with the testers.
The
nit
mutants retained pathogenicity
to
carnation.
The
F.
o.
d.
testers were not compatible with testers of five
other formae speciales
of
F.
oxysporum.
Thus,
F.
o.
d.
appears to constitute a distinct genetic population within the
F.
oxysporum
complex.
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