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Influence of chemically and biosynthesized silver nanoparticles on in vitro viability and infectivity of Trichinella spiralis muscle larvae
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Back ground
Trichinellosis is a serious worldwide parasitic zoonosis. The available therapy for the treatment of
Trichinella spiralis
is not satisfactory. This work aimed at evaluating of the in vitro effect of silver Therefore, the recovery of effective treatment is required.nanoparticles (AgNPs) on muscle larvae of
Trichinella
.
Methodology / principal finding
The present study investigated the larvicidal properties of chemical and myrrh AgNPs on muscle larvae (ML) of
T. spiralis
. The used AgNPs were chemically prepared using NaBH4 as reducing agent and biosynthesized using methanolic myrrh extract. Characterization of synthesized AgNPs was monitored via UV-Vis spectrophotometry, Fourier transform infrared spectroscopy and transmission electron microscopy (TEM) studies. The ML incubated with AgNPs at concentrations ranged from 1μg/ml to 20μg/ml.
Conclusions /Significance
Chemical and biosynthesized AgNPs revealed marked larvicidal effect against ML of
Trichinella
. Additionally, this
in vitro
study showed degenerative changes affecting the cuticle of AgNPs treated ML. The effectiveness of AgNPs on the infectivity of
Trichinella
ML was also assessed. The results showed complete inhibition of the infectivity of ML exposed to sublethal doses of chemical and myrrh prepared AgNPs when used to infect animal models. This is the first report where myrrh synthesized AgNPs have been tested for their anthelminthic activity against
Trichinella
in an
in vitro
model.
Author summary
Trichinellosis is a serious worldwide parasitic zoonosis. The available therapy for the treatment of
Trichinella spiralis
is not satisfactory. Therefore, the recovery of effective treatment is required. The present study investigated the larvicidal properties of chemical and myrrh AgNPs on muscle larvae (ML) of
T. spiralis
. The ML incubated with AgNPs at concentrations ranged from 1μg/ml to 20μg/ml. Chemical and biosynthesized AgNPs revealed marked larvicidal effect against ML of
Trichinella
. Additionally, this
in vitro
study showed degenerative changes affecting the cuticle of AgNPs treated ML. Also the results showed complete inhibition of the infectivity of ML exposed to sublethal doses of chemical and myrrh prepared AgNPs when used to infect animal models.
Title: Influence of chemically and biosynthesized silver nanoparticles on in vitro viability and infectivity of Trichinella spiralis muscle larvae
Description:
Back ground
Trichinellosis is a serious worldwide parasitic zoonosis.
The available therapy for the treatment of
Trichinella spiralis
is not satisfactory.
This work aimed at evaluating of the in vitro effect of silver Therefore, the recovery of effective treatment is required.
nanoparticles (AgNPs) on muscle larvae of
Trichinella
.
Methodology / principal finding
The present study investigated the larvicidal properties of chemical and myrrh AgNPs on muscle larvae (ML) of
T.
spiralis
.
The used AgNPs were chemically prepared using NaBH4 as reducing agent and biosynthesized using methanolic myrrh extract.
Characterization of synthesized AgNPs was monitored via UV-Vis spectrophotometry, Fourier transform infrared spectroscopy and transmission electron microscopy (TEM) studies.
The ML incubated with AgNPs at concentrations ranged from 1μg/ml to 20μg/ml.
Conclusions /Significance
Chemical and biosynthesized AgNPs revealed marked larvicidal effect against ML of
Trichinella
.
Additionally, this
in vitro
study showed degenerative changes affecting the cuticle of AgNPs treated ML.
The effectiveness of AgNPs on the infectivity of
Trichinella
ML was also assessed.
The results showed complete inhibition of the infectivity of ML exposed to sublethal doses of chemical and myrrh prepared AgNPs when used to infect animal models.
This is the first report where myrrh synthesized AgNPs have been tested for their anthelminthic activity against
Trichinella
in an
in vitro
model.
Author summary
Trichinellosis is a serious worldwide parasitic zoonosis.
The available therapy for the treatment of
Trichinella spiralis
is not satisfactory.
Therefore, the recovery of effective treatment is required.
The present study investigated the larvicidal properties of chemical and myrrh AgNPs on muscle larvae (ML) of
T.
spiralis
.
The ML incubated with AgNPs at concentrations ranged from 1μg/ml to 20μg/ml.
Chemical and biosynthesized AgNPs revealed marked larvicidal effect against ML of
Trichinella
.
Additionally, this
in vitro
study showed degenerative changes affecting the cuticle of AgNPs treated ML.
Also the results showed complete inhibition of the infectivity of ML exposed to sublethal doses of chemical and myrrh prepared AgNPs when used to infect animal models.
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