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Specific Detection of Legionella pneumophila : Construction of a New 16S rRNA-Targeted Oligonucleotide Probe
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ABSTRACT
Based on comparative sequence analysis, we have designed an oligonucleotide probe complementary to a region of 16S rRNA of
Legionella pneumophila
which allows the differentiation of
L. pneumophila
from other
Legionella
species without cultivation. The specificity of the new probe, LEGPNE1, was tested by in situ hybridization to a total of four serogroups of six strains of
L. pneumophila
, five different
Legionella
spp. and three nonlegionella species as reference strains. Furthermore,
L. pneumophila
cells could be easily distinguished from
Legionella micdadei
and
Pseudomonas aeruginosa
cells by using in situ hybridization with probes LEGPNE1, LEG705, and EUB338 after infection of the protozoan
Acanthamoeba castellanii
.
American Society for Microbiology
Title: Specific Detection of
Legionella pneumophila
: Construction of a New 16S rRNA-Targeted Oligonucleotide Probe
Description:
ABSTRACT
Based on comparative sequence analysis, we have designed an oligonucleotide probe complementary to a region of 16S rRNA of
Legionella pneumophila
which allows the differentiation of
L.
pneumophila
from other
Legionella
species without cultivation.
The specificity of the new probe, LEGPNE1, was tested by in situ hybridization to a total of four serogroups of six strains of
L.
pneumophila
, five different
Legionella
spp.
and three nonlegionella species as reference strains.
Furthermore,
L.
pneumophila
cells could be easily distinguished from
Legionella micdadei
and
Pseudomonas aeruginosa
cells by using in situ hybridization with probes LEGPNE1, LEG705, and EUB338 after infection of the protozoan
Acanthamoeba castellanii
.
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