Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Rad54 protein mediates DNA strand exchange within chromatin.
View through CrossRef
In
Saccharomyces cerevisiae
, the Rad54 protein participates in the recombinational repair of double‐strand DNA breaks (DSBs) together with the Rad51, Rad52, Rad55 and Rad57 proteins. Rad54 interacts with the Rad51 nucleoprotein filament and stimulates DNA strand exchange promoted by the Rad51 protein. Rad54 is a SWI2/SNF2‐related protein that possesses dsDNA‐dependent ATPase activity and catalyzes bidirectional nucleosome redistribution by sliding nucleosome along DNA. This chromatin remodeling activity is greatly stimulated by interaction with a Rad51 nucleoprotein filament.
Here we demonstrated that Rad54 protein efficiently mediates Rad51‐promoted DNA strand exchange within mononucleosomes, while displacing histone octamers from the dsDNA. The presence of nucleosome‐free dsDNA significantly enhances DNA strand exchange by accepting histone octamers transferred from mononucleosomes during the formation of heteroduplex DNA. The DNA strand exchange promoted by the Rad51 protein in turn stimulates the chromatin remodeling activity of Rad54. These findings indicate that the Rad54 protein facilitates chromatin remodeling at the DNA pairing and DNA strand exchange steps of genetic recombination.
Title: Rad54 protein mediates DNA strand exchange within chromatin.
Description:
In
Saccharomyces cerevisiae
, the Rad54 protein participates in the recombinational repair of double‐strand DNA breaks (DSBs) together with the Rad51, Rad52, Rad55 and Rad57 proteins.
Rad54 interacts with the Rad51 nucleoprotein filament and stimulates DNA strand exchange promoted by the Rad51 protein.
Rad54 is a SWI2/SNF2‐related protein that possesses dsDNA‐dependent ATPase activity and catalyzes bidirectional nucleosome redistribution by sliding nucleosome along DNA.
This chromatin remodeling activity is greatly stimulated by interaction with a Rad51 nucleoprotein filament.
Here we demonstrated that Rad54 protein efficiently mediates Rad51‐promoted DNA strand exchange within mononucleosomes, while displacing histone octamers from the dsDNA.
The presence of nucleosome‐free dsDNA significantly enhances DNA strand exchange by accepting histone octamers transferred from mononucleosomes during the formation of heteroduplex DNA.
The DNA strand exchange promoted by the Rad51 protein in turn stimulates the chromatin remodeling activity of Rad54.
These findings indicate that the Rad54 protein facilitates chromatin remodeling at the DNA pairing and DNA strand exchange steps of genetic recombination.
Related Results
The role of helicases and helicase-like proteins in homologous recombination
The role of helicases and helicase-like proteins in homologous recombination
Homologous recombination is important for repair of the most harmful types of DNA damage including DNA double-strand breaks, interstrand cross-links, and for chromosome segregation...
Efficient initiation and strand transfer of polypurine tract-primed plus-strand DNA prevent strand transfer of internally initiated plus-strand DNA
Efficient initiation and strand transfer of polypurine tract-primed plus-strand DNA prevent strand transfer of internally initiated plus-strand DNA
A critical step in retroviral reverse transcription is the initiation of plus-strand DNA synthesis at the polypurine tract (PPT) and strand transfer of the PPT-primed strong-stop D...
Mesoscale Modeling of a Nucleosome-Binding Antibody (PL2-6): Mono- vs. Bivalent Chromatin Complexes
Mesoscale Modeling of a Nucleosome-Binding Antibody (PL2-6): Mono- vs. Bivalent Chromatin Complexes
ABSTRACTVisualizing chromatin adjacent to the nuclear envelope (denoted “epichromatin”) by in vitro immunostaining with a bivalent nucleosome-binding antibody (termed monoclonal an...
Abstract B41: Specific blocking of miR-17-5p guide strand in triple negative breast cancer cells, without amplifying passenger strand activity
Abstract B41: Specific blocking of miR-17-5p guide strand in triple negative breast cancer cells, without amplifying passenger strand activity
Abstract
Conventional wisdom holds that only one of the two strands in a microRNA (miRNA) precursor duplex is selected as the active guide strand. The complementary ...
Abstract 3963: Antisense agents and RNA mimics for miR-17-5p guide strand and miR-17-3p passenger strand differentiate the strength of guide and passenger strand targets in PDCD4 and PTEN mRNA 3′UTRs in MDA-MB-231 triple negative breast cancer cells
Abstract 3963: Antisense agents and RNA mimics for miR-17-5p guide strand and miR-17-3p passenger strand differentiate the strength of guide and passenger strand targets in PDCD4 and PTEN mRNA 3′UTRs in MDA-MB-231 triple negative breast cancer cells
Abstract
Conventional wisdom holds that only one of the two strands in a microRNA (miRNA) precursor duplex is selected as the active guide strand. The complementary ...
Electrostatics and Solvation: Essential Determinants of Chromatin Compaction
Electrostatics and Solvation: Essential Determinants of Chromatin Compaction
ABSTRACT
Chromatin compaction is a process of fundamental importance in Biology, as it greatly influences cellular function and gene expression. The dynamics of com...
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Abstract
Background: Age-associated epigenetic alteration is the underlying cause of DNA damage in aging cells. Two types of youth-associated DNA-protection epigenetic mark...
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Introduction: The United States currently faces two opioid crises, an evolved crisis currently manifesting as widespread abuse of illicit opioids, and a crisis in pain management l...