Long Noncoding RNA Myocardial Infarction-Associated Transcript as a Cancer Promoting Factor in Colon Cancer Development

Objection: The study aimed to explain the effects and mechanisms of long noncoding RNA (lncRNA) MIAT in development of colon cancer. Methods: The adjacent and cancer tissues which were collected from 30 cases colon cancer patients were evaluated pathology by HE staining and lncRNA MIAT expression by ISH assay. In the vitro study, SW620 cells were divided into NC, siRNA-control and siMIAT groups. Measuring cell proliferation, apoptosis, cell cycle, invasion and migration by CCK-8, flow cytometry, transwell and wound healing, and evaluating the relative proteins expressions by WB assay. Results: Compared with adjacent normal tissues, the lncRNA MIAT was significantly up-regulation in colon cancer tissues (P < 0.001). By cell experiment, with lncRNA MIAT knockdown, the cell proliferation was significantly depressed (P < 0.001) via significantly improving cell apoptosis and keeping the cell in the G1 phase (P < 0.001, respectively); the invasion SW620 cell number and wound healing rate were significantly suppressed (P < 0.001, respectively). The relative proteins (CDK2, Cyclin D3 and MMP-9) expressions of siMIAT group were significantly down-regulation compared with those of NC group (P < 0.001, respectively). Conclusion: Our study found that lncRNA MIAT expression is closely associated with colon cancer and may be one of key roles in progression and metastasis in colon cancer.