Oxidation of Titanium, RGD Peptide Attachment, and Matrix Mineralization of Rat Bone Marrow Stromal Cells

AbstractThe aim of this study was to compare the efficacy of attachment of arginine-glycine-aspartic acid (RGD) peptide to titanium surfaces oxidized by different methods. Titanium surfaces were treated as follows: (1) treatment A: passivation in nitric acid, (2) treatment B: heated in air at 400°C for 1 hour, (3) treatment C: immersed in 8.8 M H2O2/0.1 M HCl at 80°C for 30 minutes, and (4) treatment D: treated as in treatment C and then heated at 400°C for 1 hour. RGD was attached to titanium samples treated as in treatments A through D. The quantity of attached RGD was determined by an enzyme-linked immunoabsorbent assay. Mineralization of a rat bone marrow stromal cell (RMSC) culture on the titanium surfaces after 21 days was determined y atomic absorption spectroscopy. The treatments were ranked according to quantity of RGD attached as C, A, B, and D. Twenty-one days after RMSC culture, the degree of mineralization was significantly higher for treatment C than for treatments A, B, and D and for controls. The efficacy of RGD attachment varies with the oxidation treatment given to titanium. Oxidation in H2O2/0.1 M HCl at 80°C provided the best overall surface for RGD attachment as well as calcified matrix formation of RMSCs.