Supplementary Figures from E7386, a Selective Inhibitor of the Interaction between β-Catenin and CBP, Exerts Antitumor Activity in Tumor Models with Activated Canonical Wnt Signaling

<p>Supplementary Figures 1-9. Supplementary Fig. S1 Wnt/β-catenin-dependent reporter activity in ECC10 cells. Supplementary Fig. S2 Effect of E7386 on the interaction between β-catenin and p300 in ECC10 cells. Supplementary Fig. S3 Comparison of IC50 values among β-catenin/CBP inhibitors, as determined by HEK-293 TCF/LEF reporter assay. Supplementary Fig. S4 Upstream regulators of gene expression changes in whisker follicle tissues of ApcMin/+ mice treated with E7386. Supplementary Fig. S5 Inhibitory activity of E7386 against cell growth in ECC10 cells. Supplementary Fig. S6 Antitumor activity of E7386 and ICG-001 in a human ECC10 gastric tumor xenograft model. Supplementary Fig. S7 Gating strategy for the flow cytometry analysis of myeloid and T-cell populations. Supplementary Fig. S8 Flow cytometry analysis of the effect of E7386 on immune cell populations in the MMTV-Wnt1 model. Supplementary Fig. S9 Effect of PRI-724 on spontaneous intestinal polyp formation in ApcMin/+ mice.</p>