Intestinal Enzymes and Lactid Acid Bacteria of Red Tilapia (Oreochromis sp.) Fed Black Soldier Fly (Hermetia illucens) Larvae and Probiotics

This study aimed to determine the effect of probiotics on the intestinal digestive enzyme, morphology and total bacteria of red tilapia (Oreochromis sp.) fed maggot BSF (Hermetia illucens) larvae. The research was conducted using randomized design of two treatments (with probiotics and without probiotics) in three replications. Tilapia (average weight 70 g) were reared for 60 days with diet composition of commercial feed and black soldier fly (Hermetia illucens) larvae at 7:3, respectively. Oral probiotics (Lactococcus sp. JAL 37 and Bacillus sp. PCP 1 (107 cell/g feed) and water probiotic (Klebsiella sp. A2, 105 cell/ml) were administered in seven days interval. We found that administration of maggot BSF (Hermetia illucens) larvae provide no defects on the fish gut morphology. The probiotics application did not change the intestinal cellulose enzyme activity, as well as the intestinal histology parameters, such as villi length, muscular layer thickness and amount of goblet cells. However, the probiotic application significantly enhanced protease and lipase activity (p<0,05), reduced total number of Aeromonas and enhanced total number of lactic acid bacteria (p<0,05).