Adverse Effect of Rheumatoid Arthritis on Male Wistar Rat’s Fertility: Protective Role of Costus Extract

Abstract Rheumatoid arthritis (RA) is an autoimmune systemic complaint. Costus speciosus, an ornamental plant, which traditionally used in urinary diseases, rheumatism, jaundice and dropsy. The purpose of the study is to assess the protective effect of Costus on rheumatoid arthritis induced male rat in fertility. Thirty male adult Wistar rats (190-200 g) were sectioned into 6 groups. They were subdivided into 3 groups; group 1 was control received Distilled water, groups II and III received two various doses of Costus extract (200 and 400 mg/kg, respectively) for 60 days. Another 3 groups were subjected to RA induction via Freund's adjuvant. First group of RA induced rats was given Distilled water. Other two groups were given orally (200 and 400 mg/kg dosage of extract, respectively) from the 2nd day of RA induction for 60 days. Sex organ relative weight, sperm concentration assay, testicular histopathology and immunohistochemistry of androgen receptors, TNF α and Bax protein were determined. Rheumatoid arthritis caused significant decrease in relative weight of sex organs which relatively improved by doses of Costus (200, 400 mg/kg). In addition, RA caused a significant reduction in sperm count which was improved via Costus (200, 400 mg/kg). RA induction caused testicular degeneration with marked depletion of spermatozoa, which markedly improved with costus treatment as shown in histopathological sections. Rheumatoid arthritis caused a reduction in %IHC of androgen receptors and increase of %IHC of both TNF α and Bax protein. Costus (200, 400 mg/kg) significantly improved %IHC of androgen receptors and significantly decreased %IHC of both TNF α and Bax protein, in RA induced groups. We can concluded that Costus speciosus had potentially useful role in improving fertility disorders caused by RA.