Tofu is a food that contains protein and is made from soybean juice obtained through the fermentation process of soybeans. An increase in tofu consumption results in an increase in energy demand in a tofu processing industry. Energy use that is carried out efficiently can be done by means of an energy audit that will get the energy used and know how to save it. This study aims to 1) determine the energy requirements of each tofu processing process, 2) determine the form, source and amount of energy used in the tofu processing production process in Sampang Village and Brani Village, Sampang District, Cilacap Regency, Central Java, 3) find out the comparison of the efficiency levels of the two tofu processing industries in Sampang Village and Brani Village, Cilacap District, Central Java, and 4) identify the results of the comparison of data obtained from the two tofu processing industries to make an effort to save energy or diversify energy use. This research was conducted on two small industries in Sampang Village owned by Mr. Maryo and Mr. Sholeh's Brani Village located in Sampang District, Cilacap Regency, Central Java. This research was carried out from February 2021 to April 2021. The sampling was carried out on these two industries in order to obtain energy data and observe the differences in the tools used when processing the tofu. The data collection method is carried out for each production in one day. Variables obtained from data collection include the amount of tofu production per day, energy requirements for biomass fuels (rice husks and firewood), human energy needs, electrical energy requirements, diesel fuel energy requirements, and energy requirements for ballast stones.  The results showed that the energy needed in the tofu processing industry Mr. Maryo was 779.06 MJ or 5.19 MJ/kg obtained from energy sources such as human energy (33.47 MJ or 0.22 MJ/kg), biomass fuel energy (552, 29 MJ or 3.68 MJ/kg), and diesel fuel energy (193.30 MJ or 1.29 MJ/kg).The energy used in the tofu processing industry belonging to Mr. Maryo is 244.81 MJ or 1.63 MJ/kg which comes from the energy used in this industry such as human energy (18.43 MJ or 0.12 MJ/kg), biomass fuel energy (122.54 MJ or 0.82 MJ/kg), and diesel fuel energy (103.84 MJ or 0.69 MJ/kg). The results of research on the tofu processing industry of Mr. Sholeh amounted to (2205.77 MJ or 6.302 MJ/kg) obtained from energy sources such as human energy of (33.47 MJ or 0.10 MJ/kg), biomass fuel energy (2109.60 MJ or 6,03 MJ/kg), and electrical energy (62.70 MJ or 0.18 MJ/kg). The energy used in Sholeh's tofu processing industry (298.74 MJ or 0.86 MJ/kg) comes from energy used in industries such as human energy (32.22 MJ or 0.09 MJ/kg), biomass fuel energy (214.11 MJ or 0.61 MJ/kg), and electrical energy (52.41 MJ or 0.15 MJ/kg).