DESIGN AND ANALYSIS OF CISSUS QUADRANGULARIS L. BY DESIGN OF EXPERIMENTS AS A NOVEL HERBAL GEL

Cissus quadrangularis has a common Bengali name as HARJORA has the property of BONE-HEALING. Using DESIGN OF EXPERIMENTS, it was formulated a novel Gel with Propylene Glycol and Carbopol 940 as Independent-Variable and Viscosity, Spreadibility Factor and Endurability as Dependable-Variable. Thus, a Factorial Design by DOE as 3 to the power 2, or 32 or 9 nine Formulations. An optimum formulation was obtained as F-1 from a series of F-1 to F-9. Out of these nine formulations tested for Quality Control F-1 depicts the gem of nine. In case of a crack or fracture F-1 can be used to heal the bone by applying locally. In DOE factorial design, -1,0, & +1 Level for Propylene Glycol [PG] and Carbopol 940 were taken for experimentation purpose. With 1:1 EtOH:H2O as a solvent, the green CQ Plant modified stem was dissolved 3 times of the macerant. After filtration and centrifugation the dose was adjusted in 5ml of the extract. An elaborate study by varying the ingredients formulations were prepared. These formulations were undergone analysis by Macro-Physico established protocols. Viscosity, Spreadibility, Extrudability, Conductivity and pH homogeneity were among the parameters. In future, stability indicating protocols to be developed that will assure the shelf-life of this Harjora-Herbal-Gel.