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Primary structure of a cytotoxin‐like basic protein from Naja naja naja (Indian Cobra) venom
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The complete amino acid sequence of a cytotoxin‐like basic protein (CLBP) from the venom of Naja naja naja (Indian Cobra) was determined by manual degradation using a 4‐dimethylaminoazobenzene‐4′‐isothiocyanate double‐coupling method. Peptide fragments obtained by chemical cleavage with cyanogen bromide and enzymic cleavages with trypsin and Staphylococcus aureus proteases for sequence analysis were purified by reversed‐phase chromatography. The total number of amino acid residues was 61, with leucine as the C‐terminal residue.
Title: Primary structure of a cytotoxin‐like basic protein from Naja naja naja (Indian Cobra) venom
Description:
The complete amino acid sequence of a cytotoxin‐like basic protein (CLBP) from the venom of Naja naja naja (Indian Cobra) was determined by manual degradation using a 4‐dimethylaminoazobenzene‐4′‐isothiocyanate double‐coupling method.
Peptide fragments obtained by chemical cleavage with cyanogen bromide and enzymic cleavages with trypsin and Staphylococcus aureus proteases for sequence analysis were purified by reversed‐phase chromatography.
The total number of amino acid residues was 61, with leucine as the C‐terminal residue.
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