Single‐anastomosis duodeno‐ileal bypass with sleeve gastrectomy improves lipid and glucose homeostasis in ob/ob mice

AbstractObjectiveThe objective of this study was to compare the general and metabolic impact of single‐anastomosis duodeno‐ileal bypass with sleeve gastrectomy (SADI‐S) with Roux‐en‐Y gastric bypass (RYGB) in an obese (ob/ob) mouse model.Methods10‐week‐old male ob/ob mice underwent either SADI‐S, RYGB, or laparotomy surgery (Sham group). General and metabolic parameters were assessed during a 5‐week period thereafter.ResultsSADI‐S induced a deeper weight loss ([mean ± SEM] −41.2% ± 3.3%) than RYGB (−5.6% ± 3.5%, p < 0.001) compared with the Sham group (+6.3% ± 1.0%, p < 0.05). A significant food restriction was observed after SADI‐S only (−31%, 117.4 ± 10.3 g vs. 170.2 ± 5.2 g of food at day 35 in Sham group mice, p < 0.001). Random‐fed glycemia and glucose tolerance were more improved after SADI‐S than RYGB. SADI‐S decreased plasma cholesterol concentration by 60% (0.49 ± 0.04 g/L vs. 1.40 ± 0.10 g/L in the Sham group at day 35, p < 0.01), significantly more than RYGB (1.04 ± 0.14 g/L, p = 0.018). Plasma sitosterol/cholesterol and campesterol/cholesterol ratios were decreased after SADI‐S, suggesting a reduced intestinal cholesterol absorption. SADI‐S increased exogenous plasma cholesterol‐D7 clearance and fecal elimination, also indicating an increased plasma cholesterol excretion. Studying a pair‐fed group demonstrated that calorie restriction alone did not explain the beneficial impact of SADI‐S.ConclusionsSADI‐S is associated with a greater improvement in lipid and glucose homeostasis than RYGB in ob/ob mice.