Development of novel wound dressing based collagen/chitosan/nigella sativa on blood leukocyte profile and malondialdehyde levels in incision wounds

Introduction: Nigella sativa for wound dressing has never been developed already. It could be incorporated into collagen and chitosan as composite dressing to accelerate wound repair. This study aimed to analyze the effect of collagen/chitosan/Nigella sativa composite dressing for incision wound care in differential leukocyte count and serum malondialdehyde (MDA) levels. Methods: Collagen/Chitosan/Nigella sativa composite dressing was synthesized by mixing collagen and chitosan solution with Nigella sativa oil in various concentrations, which were divided into four main groups such as N (1:1:0 w/w/w), IL (2:2:1 w/w/w), IM (1:1:1 w/w/w), and IH (1:1:2 w/w/w). Leukocyte profiles, including basophile, eosinophil, neutrophil stabs, neutrophil segments, lymphocytes, and monocytes, were observed using blood smear methods on the 3rd and 14th days. A serum malondialdehyde (MDA) assay was conducted using a UV-VIS spectrophotometer. Results: Differential leukocyte count decreased from the 3rd and 14th days, except lymphocyte count, which continued higher on the 14th day. The serum MDA assay revealed decreased levels from the 3rd and 14th days. Conclusion: Incision wound care using collagen/chitosan/Nigella sativa dressing could reduce the inflammation phase and accelerate the wound healing process.