Comparative therapeutic effectiveness of probiotic and combinations of Probiotic with prebiotics and probiotic with rumenotorics in experimentally induced ruminal acidosis sheep

Abstract Backgroundrumen disorder is responsible for huge economic losses in ruminants due to decrease in production and increase in expenditure towards treatment. Acidosis is one of the most ruminal disorders, characterized by changes in the rumen environment and leds to systemic acidosis. To correct this disorder, recent alternative trends in sheep rearing have led to the use of prebiotics, rumenotorics and probiotics as feed additives or treatment.Context and purposethis study was to evaluate and compares the therapeutic effectiveness of probiotics, a combination of probiotics with prebiotics, probiotics with rumenotorics and with the standard treatment under experimentally induced acidosis.Methodsthe experimental study design was conducted from September to May 2019. For therapeutic study 25 sheep were randomly divided into 5 groups with deliberately imposed ruminal acidosis as a group comprising of 5 animals each.Resultsacidosis was induced by an oral dose of 50 g/kg with wheat flour after 24-hour fasting. Four therapeutic regimes and negative control group were employed, I-Probiotics, II-Probiotics with Prebiotics, III-Probiotics with Rumenotorics and IV-Standard treatment (sodium bicarbonate) was adopted. Then, laboratory analysis of rumen fluid, physical parameters and hematological changes before and after treatment was carried out. From a comparison of each treatment groups with the control group to correct ruminal pH, the combination of probiotics with rumenotorics, the mean ± SD before treatment (4.96 ± .837) increased to (5.92 ± .54), (6.30 ± .41), (6.75 ± 0.34) after treatment, was statistically significant (p < 0.05, 0.024, 0.047, 0.002), from day one-day three, respectively, and the therapeutic regimens of probiotics with rumenotorics was highly significance compare with control group (p < 0.05, p = 0.001 & p = 0.000) improved heart rate and respiratory rate at day three after treatment and the mean PCV value of treatment Group III was 38.00 ± 6.819 before treatment changed to 34.60 ± 5.367, 29.80 ± 6.261 and 24.80 ± 10.134 after treatment, were statistically significance (p < 0.05, p = 0.003, p = 0.02 &0.006) from day one-day three, respectively.Conclusionthe present study was concluded that probiotics with rumenotorics administered together were the most successful therapeutic regimen for improvement of ruminal acidosis. Therefore, in this experimental study for the treatment of acidosis, it has been recommended that use of probiotics with substrate is the promising alternative.