Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Molecular Characterization of Cytokinin-Responsive Histidine Kinases in Maize. Differential Ligand Preferences and Response to cis-Zeatin
View through CrossRef
AbstractGenes for cytokinin-responsive His-protein kinases (ZmHK1, ZmHK2, and ZmHK3a) were isolated from maize (Zea mays). Heterologous expression of each of the ZmHKs in Escherichia coli having the ΔrcsC and cps∷lacZ genetic background conferred cytokinin-inducibility of lacZ expression on the bacteria. In the recombinant E. coli system, ZmHK1 and ZmHK3a were more sensitive to free-base cytokinins than to the corresponding nucleosides; isopentenyladenine was most effective for ZmHK1, while ZmHK2 tended to be most sensitive to trans-zeatin and the riboside. In contrast to a known cytokinin receptor of Arabidopsis (AHK4/CRE1/WOL), all ZmHKs responded to cis-zeatin (cZ), which generally is believed to be inactive or only weakly active. In cultured maize cells, expression of ZmRR1, a cytokinin-inducible response regulator, was induced by cZ as well as by trans-zeatin. These results strongly suggest that maize cytokinin receptors differ in ligand preference, and that cZ is an active cytokinin at least in maize.
Oxford University Press (OUP)
Title: Molecular Characterization of Cytokinin-Responsive Histidine Kinases in Maize. Differential Ligand Preferences and Response to cis-Zeatin
Description:
AbstractGenes for cytokinin-responsive His-protein kinases (ZmHK1, ZmHK2, and ZmHK3a) were isolated from maize (Zea mays).
Heterologous expression of each of the ZmHKs in Escherichia coli having the ΔrcsC and cps∷lacZ genetic background conferred cytokinin-inducibility of lacZ expression on the bacteria.
In the recombinant E.
coli system, ZmHK1 and ZmHK3a were more sensitive to free-base cytokinins than to the corresponding nucleosides; isopentenyladenine was most effective for ZmHK1, while ZmHK2 tended to be most sensitive to trans-zeatin and the riboside.
In contrast to a known cytokinin receptor of Arabidopsis (AHK4/CRE1/WOL), all ZmHKs responded to cis-zeatin (cZ), which generally is believed to be inactive or only weakly active.
In cultured maize cells, expression of ZmRR1, a cytokinin-inducible response regulator, was induced by cZ as well as by trans-zeatin.
These results strongly suggest that maize cytokinin receptors differ in ligand preference, and that cZ is an active cytokinin at least in maize.
Related Results
ANTIPROLIFERATIVE ACTIVITIES Of EXTRACTS FROM MYCELIAL BIOMASS OF SOME MEDICINAL BASIDIOMYCETES IN HUMAN COLON CANCER CELLS COLO 205
ANTIPROLIFERATIVE ACTIVITIES Of EXTRACTS FROM MYCELIAL BIOMASS OF SOME MEDICINAL BASIDIOMYCETES IN HUMAN COLON CANCER CELLS COLO 205
Background: The anticancer effects of phytohormones of cytokinin nature are similar to those of medicinal mushrooms, which are able to synthesize cytokinins in large amounts. Aim: ...
Copper indium sulfide colloidal quantum dot solar cells
Copper indium sulfide colloidal quantum dot solar cells
The ubiquity of solar energy conversion technology is an exciting target which we aspire for in this century. Colloidal quantum dot (CQD) solar cells are an attractive platform, be...
Push-pull cropping system soil legacy alter maize metabolism and fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) resistance through tritrophic interactions”
Push-pull cropping system soil legacy alter maize metabolism and fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) resistance through tritrophic interactions”
Abstract
Background and aims
Crop cultivation practices and soil legacies are intrinsically linked and are hypothesized to influence plant direct and indirect defence again...
Improvement of Provitamin A in Maize Varieties Using Arbuscular Mycorrhizal Fungus, Glomus clarum
Improvement of Provitamin A in Maize Varieties Using Arbuscular Mycorrhizal Fungus, Glomus clarum
Arbuscular mycorrhizal fungus (AMF, Glomus clarum) has been used widely as a bio-amendment and bio-control agent in several biotechnological studies. In this study, biofortificatio...
AGRONOMIC PERFORMANCE AND PHOTOSYNTHETICALLY ACTIVE RADIATION INTERCEPTED BY MAIZE INTERCROPPED WITH BRACHIARIA
AGRONOMIC PERFORMANCE AND PHOTOSYNTHETICALLY ACTIVE RADIATION INTERCEPTED BY MAIZE INTERCROPPED WITH BRACHIARIA
Maize and soybean are the main crops used for crop yield on Brazilian Cerrado biome. The off-season maize intercropped with ruzigrass [Urochloa ruziziensis (R. Germ. & C.M. Evr...
Response of Soybean (Glycine max (L.) Merr.) and Weed Control with Postemergence Herbicides and Combinations of Cytokinin Mixtures
Response of Soybean (Glycine max (L.) Merr.) and Weed Control with Postemergence Herbicides and Combinations of Cytokinin Mixtures
A field study was conducted in 2015 and 2016 in Stoneville, MS, to evaluate the influence of cytokinin products on soybean injury and weed control when combined with common POST so...
Genome-wide identification of the soybean cytokinin oxidase/dehydrogenase gene family and its diverse roles in response to multiple abiotic stress
Genome-wide identification of the soybean cytokinin oxidase/dehydrogenase gene family and its diverse roles in response to multiple abiotic stress
Cytokinin oxidase/dehydrogenase (CKX) irreversibly degrades cytokinin, regulates growth and development, and helps plants to respond to environmental stress. Although the CKX gene ...
Occurrence of aflatoxigenic fungi and aflatoxins in maize grains and associated awareness and handling practices among farmers and traders in South Sudan
Occurrence of aflatoxigenic fungi and aflatoxins in maize grains and associated awareness and handling practices among farmers and traders in South Sudan
Maize is a popular staple food among the urban population of South Sudan. However, due to inadequate food safety surveillance and enforcement systems, there is limited information ...