Biological recultivation of soils contaminated with fuel oil, using vermiculture earthworms Dendrobena veneta and microbiological preparations «Baikal-Em», «Tamir», «East»

The technology of soil remediation contaminated with fuel oil at a concentration of 150 g/kg of soil for one year in Western Siberia using microbiological preparations and earthworms was developed. Earthworms have great potential for removing hydrocarbons from the soil, even at high concentrations up to 100 g/kg. The introduction of microbiological preparations increased the survival and reproductive potential of worms in oil-contaminated soils and contributed to a significant reduction in the concentration of hydrocarbons of fuel oil for 20–22 weeks in the laboratory and field experiment. These results suggest that earthworms are effective in bioremediation of even very contaminated soils with a concentration of fuel oil up to 100 g/kg when combined with microbiological preparations containing cultures of bacteria Paenibacillus pabuli, Azotobacter vinelandii, Lactobacillus casei, Clostridium limosum, Cronobacter sakazakii, Rhodorulla mucilaginosa, Cryptococcus albidus, yeast Saccharomyces, Candida lipolitica, Candida norvegensis, Candida guilliermondii, fungi Aspergillus and Penicillium as well as actinomycetales. (CFU )=2*1011 microorganisms per ml a method of remediation of oil-contaminated soils with fuel oil content up to 150 g/kg by microorganisms (lactic acid, nitrogen-fixing, photosynthetic bacteria) and earthworms Eisenia andrei is Proposed. A method for cleaning and restoring the environmental functions of substrates contaminated with fuel oil and other petroleum products, was that the substrate was treated with biologics, carried out dump soil treatment and steamed for one month, then introduced earthworms in an amount of 1000 PCs./m2, as a nutrient medium used cattle manure (cattle) in a dose of 1 t/ ha of contaminated landfill.