Dynamics of mitochondrial nucleoid proteins involved in development of cotyledon mitochondria during mung bean seed germination

Abstract Mitochondrial DNA (mtDNA) protein complexes, called mitochondrial-nucleoids (mt-nucleoids), are dynamic and redistribute during mitochondria propagation and gene expression. These proteins involve in mtDNA replication, transcription, maintenance and repair. In this study, we analyzed mt-nucleoid proteins in isolated mt-nucleoids from cotyledons at different developmental ages. We aimed to understand the changes in mt-nucleoids associated protein during cotyledon development. We found that some transcription/translation factors pre-exist in nucleoids of dormant seed mitochondria. High numbers of metabolic proteins co-purified with mtDNA appeared in dormant seeds and developmental cotyledon mitochondria. This report provides some idea about the dynamic of mt-nucleoid proteins along with cotyledon mitochondrial development during seed germination.