Nanoemulsions Containing Passiflora quadrangularis L. Fruit Extracts for Cosmetic Application and Skin Efficacy Study

This study aimed to determine the anti-collagenase, anti-elastase, and anti-hyaluronidase activities of Passiflora quadrangularis fruit extracts (epicarp, mesocarp, endocarp, and seed), develop stable nanoemulsions, and evaluate the efficacy of the nanoemulsions containing extracts in human volunteers. The results indicated that the epicarp and seed extracts exhibited collagenase, elastase, and hyaluronidase inhibition effects. Gallic acid was identified in the extracts, with the highest concentration found in the endocarp extract (1449.35 mg gallic acid/g extract) followed by the seed extract (839.63 mg gallic acid/g extract). The endocarp and seed extracts demonstrated good stability at different temperatures. Consequently, these extracts were selected for incorporation into nanoemulsions due to their high yield, excellent anti-tyrosinase and anti-aging activity, and good stability, making them suitable for cosmetic products. The nanoemulsions were prepared using the ultrasonication method, resulting in a particle size of 133.1 ± 0.8 nm, a polydispersity index of 0.33 ± 0.03, and a zeta potential of −61.8 ± 0.6 mV, indicating good stability. The nano-serum containing extracts was non-irritating and safe for the skin. Skin evaluation among human volunteers after 60 days of application revealed improvements in skin hydration and reduction in wrinkles. In conclusion, nanoemulsions containing P. quadrangularis fruit extracts have the potential to be effective cosmetic products.