Structure Prediction of the Bombyx mori Sericin 4 Protein

Natural silk (Bombyx mori) has been found to contain sericin 1, sericin 2, sericin 3, and sericin 4 proteins. The sequence of amino acid residues in them has also been well studied. However, there is little information on the molecular structure of sericin 4. We conducted studies on the prediction of the sericin 4 molecule's structure using the AlphaFold3 and YASARA computational servers. Molecular dynamics simulations were performed in aqueous solution to evaluate the stability and determine the most favourable conformation of the predicted sericin 4 structure. We mainly used the ProSA-web, Ramachandran Z and Molprobity score to evaluate the predicted structure of sericin 4, and the reliability of the predicted model was determined. The predicted molecular structure serves as a preliminary, yet robust, model of sericin 4.