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Development and Application of a Filamentous Phage-Based Rapid Detection Tool for Ralstonia pseudosolanacearum
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Abstract
Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is a devastating plant disease with a broad host range. Early detection is critical for disease management, yet conventional methods lack speed and specificity. This study developed a rapid detection system using engineered filamentous phages (RSCq) expressing bioluminescence genes (luxAB, nanoluc, and luxSit-i). Among the constructs, RSCqluxAB demonstrated optimal performance, detecting R. pseudosolanacearum at 1.58×103 CFU/mL within 12 hours, with minimal background noise. The phage retained stability for six months at 4°C, proving suitable for field applications. These findings highlight its potential for early pathogen monitoring, quarantine enforcement, and precision agriculture, though further validation in soil/plant samples is needed.
Title: Development and Application of a Filamentous Phage-Based Rapid Detection Tool for Ralstonia pseudosolanacearum
Description:
Abstract
Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is a devastating plant disease with a broad host range.
Early detection is critical for disease management, yet conventional methods lack speed and specificity.
This study developed a rapid detection system using engineered filamentous phages (RSCq) expressing bioluminescence genes (luxAB, nanoluc, and luxSit-i).
Among the constructs, RSCqluxAB demonstrated optimal performance, detecting R.
pseudosolanacearum at 1.
58×103 CFU/mL within 12 hours, with minimal background noise.
The phage retained stability for six months at 4°C, proving suitable for field applications.
These findings highlight its potential for early pathogen monitoring, quarantine enforcement, and precision agriculture, though further validation in soil/plant samples is needed.
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