HISTOMORPHOLOGICAL EFFECTS OF SALICYLIDENE SALICYL HYDRAZIDE ON LIVER IN SPRAGUE DAWLEY RATS

Objective: This study was aim to identify histomorphological changes in the liver of Sprague dawley rats after exposure to Saliyclidene salicylhydrazide and dose dependent histomorphological changes in the liver. Materials and Methods: This study was started from Mar 2019, to June 2019. In this study, acute toxicity of seven days, the sixteen Sprague dawley female rats were selected. They were divided into four groups. Two test groups (A & B). Group A was given 25mg/kg SCS, while in group B 50mg/kg of SCS was given. Positive control group (C) was given paracetamol 300mg/kg. Group D was a negative control group. Each group had four animals. After seven days of exposure to SCS through oral route all the animals were euthanized. Their livers were harvested and processed for preparation of histological slides. These slides were then analyzed by microscopy and histomorphological changes were recorded and analyzed according to Roenigk classification system. Results: Necroinflammatory changes were observed in Group A, B and C compared to Group D(-control). However, difference between Group A and B was not significant. Nuclear pleomorphism was observed in hepatocytes of Group A, B and C compared to group D(control). Pleomorphic changes in the intervention group A & B were same. Steatosis was observed in group A, B and C compared to Group D. Steatosis in group A and B were same while in group C it was relatively more significant. The overall grading according to ROENIGK classification system showed significantly higher toxicity in group B, compared to other intervention groups (A, B, C) and control group D. Conclusion: Significant effects of SCS were observed in liver of Sprague dawley rats, however, the toxic effects were less severe. Liver Parenchyma and architecture were preserved.