A Computational Method for detecting and evaluating Tankyrase-Binding Motifs v1

Tankyrases are multifunctional proteins within the poly(ADP-ribose) polymerase family. Known tankyrase binders primarily interact with the scaffolding portion of tankyrases, which comprises five ankyrin repeat cluster (ARC) domains. These domains recognize a specific sequence known as the Tankyrase binding motif (TBM), typically following an octapeptide format characterized by an arginine at position 1 and a glycine at position 6. However, extended and nonconventional TBMs have also been reported. At present, there is no system in place that can easily find and score TBMs. This protocol describes how to use a web-based, public-accessible, computational method (https://shellmanlab.github.io/) we developed to locate and rank all types of potential TBMs. As interest in tankyrases continues to grow across various biological fields, our tool empowers researchers to quickly assess how tankyrases may impact their protein of interest.