Potential Impacts of Dried Green Coleus forskolin Leaves on Thermo-tolerance Parameters, Blood and Plasma Profiles in Anesthetized Mice

Background: Transient negative side effects of hypothermia, hyperglycemiaand bradycardia due to general anesthesia using diazepam and xylazine drugs has been reported in mice. Methods: Twenty albino male mice of 31.52±0.25 g body weight were classified into two groups; control group (G1) fed basal control diet versus C. forskolin group fed basal control diet containing C. forskolin leaves (G2; 1.5%) for four weeks. The mice were general anesthetized using xylazine and diazepam (XD) drugs (13.3 mg/kg BW diazepam and 26.6 mg/kg BW xylazine). Changes of rectal temperature (°C), glucose (mg/dl), pulse rate (pulse/min.) and partial pressure of oxygen (SPO2) were recorded at 0.0, 0.5h, 1.0h, 2.0h, 3.0h, 5.0h and 7.0h over general anesthesia. In addition, blood samples were collected and were subjected to blood and plasma analyses. Result: The results indicated that dietary C. forskolin caused significant decrease (P less than 0.05) in glucose values in addition to significant increase (P less than 0.05) in rectal temperature, pulse rate and SPO2 during the period hypothermia, hyperglycemiaand bradycardia over general anesthesia. In addition, values of blood (RBCs, WBCs, PCV and Hb) and plasma parameters (TP, BUN, creatinine, AST and ALT, LDH) were normal due to C. forskolin supplementation compared to control diet. It could be concluded that 1.5% C. forskolin supplementation modulates thermo-tolerance responses, blood glucose indices, blood and plasma metabolites over general anesthesia.