Clinical value of lncRNA CARMN expression in threatened abortion and its effect on cell function of HTR-8/SVneo

Abstract Objectives Threatened abortion (TA) is a common condition in pregnant women. The role of CARMN in the onset and progression of TA is increasingly recognized as significant. However, the precise mechanisms of its influence are not yet fully understood. This study was to investigate the clinical value of CARMN in TA patients and the mechanism of action that may influence TA. Methods This study included 105 patients aged 22–38 years old, 6–8 weeks pregnant and with early TA of single pregnancy. In addition, 63 healthy women of the same age group who were 6–8 weeks pregnant were included as controls. qRT-PCR was employed to assess the expression levels of CARMN and miR-515-5p. The clinical diagnostic utility of CARMN in patients with TA and the predictive value of CARMN in pregnancy outcome of TA patients were analyzed using ROC curve methodology. Cell viability in HTR-8/SVneo cells was measured utilizing a cell counting kit-8 (CCK-8) kit. Apoptosis rates were quantified through flow cytometry. Cell migration was investigated using a transwell assay. Results The level of serum CARMN decreased and miR-515-5p increased in patients with TA. CARMN could predict pregnancy outcomes in patients with TA. Functionally, CARMN enhanced the viability and migration of HTR-8/SVneo cells by regulating the expression of miR-515-5p, while inhibiting apoptosis. Conclusions LncRNA CARMN may serve as a diagnostic marker for TA and participate in the progression of TA by regulating the expression of miR-515-5p.