PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF WHOLE PLANT OF TRIDAX PROCUMBENS LINN.

Antioxidants serve a critical role in preventing oxidative stress-related damage. Plants with high phenolic content havebeen shown to have antioxidant effects. The goal of this study was to look into the phytochemistry and antioxidantproperties of ethanolic extracts from Tridex procumbens (whole plant) in order to establish a statistical link betweenisolated component concentration and antioxidant capability. TLC, HPTLC, Column chromatography, and an in vitrostandard technique employing a spectrophotometer were used to assess the phytochemistry and antioxidant properties ofethanolic extractives. DPPH (l, l-diphenyl-2-piciylhydrazine) radical scavenging assay, Nitric oxide (NO) RadicalInhibition Assay, Reducing Power and hydroxyl radical scavenging assay techniques were used to assess antioxidantactivity. TLC plate with various numbers of 5 spots were implemented. The HPTLC report shows the existence of tenspots with distinct Rf values, indicating that they occupy separate areas with varying Rf values. The extracted chemicalsfrom the ethanolic extract of Tridex procumbens were column chromatographed using hexane (80ml) and benzene (20 ml)and a stationary phase silica gel. The maximum antioxidant activity was found in extracts. The Tridax procumbens extractwas the most efficient in terms of DPPH and hydroxyl radical scavenging activities, with IC50 values of 0.15, 0.12, 0.008and 42.39 g/ml, respectively. The findings show a strong link between separated chemical content and extractantioxidant capability, suggesting that Tridex procumbens might be a promising option for medicinal plant-based products asa free radical inhibitor or scavenger. However, further research is needed to ensure that it can be used effectively in bothmodern and traditional medicine.