In-Vitro Analysis of Murraya Koenigii Root extract with Colistin Against MDR Pseudomonas Aeruginosa

Objectives: To analyze susceptibility of MDR Pseudomonas aeruginosa to chloroform root extract ofMurraya koenigii.Methodology: For this research Murraya koenigii chloroform root extract (MKCRE) was obtained from PCSIR, Lahorewith total yield of 3.4%. A 14-day study was conducted on blood samples of 12 patients admitted to Sheikh ZayedHospital (SZH) Lahore over a period of one year, assessed positive for MDR P. aeruginosa based on a series ofconfirmatory bench tests performed in the Microbiology lab of SZH Lahore. Simple Randomized sampling was doneby rotary method, and the samples were randomly divided into 2 groups, each group having 6 samples. Groups 1 & 2were tested for susceptibility to MKCRE and Colistin respectively using Disk diffusion and Agar well diffusion methodsfor determination of zone of inhibition and MIC (Minimum Inhibitory Concentration) was determined by brothmacro-dilution method. Data was statistically analyzed using SPSS version 25.Results: Our study concluded that MKCRE have significant anti MDR P. aeruginosa activity with pharmaceuticalpotential.Conclusion: The current study revealed that Murraya koenigii chloroform root extract individually carries Anti MDRPseudomonal potential is better than Colistin.Keywords: Murraya koenigii root extract, Colistin, Pseudomonas aeruginosa