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NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546) v1
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The NEBNext Ultra II End Repair/dA-Tailing Module is optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5 ́ phosphorylated, 3 ́ dA-tailed ends. This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext™ Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction. This module is also compatible with some Oxford Nanopore MinION™workflows. This module is designed for use with NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535), NEBNext Ultra II Ligation Module (NEB #E7595), and NEBNext Ultra II Q5 Master Mix (NEB #M0544). Kits that include reagents for every step in the Ultra II DNA library construction workflow are also available (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103).
Title: NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546) v1
Description:
The NEBNext Ultra II End Repair/dA-Tailing Module is optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5 ́ phosphorylated, 3 ́ dA-tailed ends.
This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext™ Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction.
This module is also compatible with some Oxford Nanopore MinION™workflows.
This module is designed for use with NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600 or #E7535), NEBNext Ultra II Ligation Module (NEB #E7595), and NEBNext Ultra II Q5 Master Mix (NEB #M0544).
Kits that include reagents for every step in the Ultra II DNA library construction workflow are also available (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103).
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