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Optimization of the Cultivation Processof a Producer Clone of the Recombinant Lysosomal Iduronate-2-sulfatase Enzyme
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This paper describes experiments aimed at developing conditions for suspension culturing of a producer clone of the recombinant lysosomal enzyme iduronate-2-sulfatase based on the CHO cell line. As a result of sequential culturing in batch and fed-batch modes, the optimal nutrient medium composition was established, providing a 2.5-fold increase in the iduronate-2-sulfatase specific activity. Optimization of the feed formulation was carried out, which increased the yield of recombinant iduronate-2-sulfatase. The ambr® 15 Cell Culture System of minibioreactors was used to optimize the cultivation process. Chinese hamster ovary cells, CHO, iduronate-2-sulfatase, idursulfase, lysosomal enzyme, sulfatases, Аmbr Tap Biosystems, ambr® 15 Cell Culture System, mucopolysaccharidosis type II, formyl glycine generating enzyme, optimization of cell culturing conditions, fed-batch culture, batch culture.
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Title: Optimization of the Cultivation Processof a Producer Clone of the Recombinant Lysosomal Iduronate-2-sulfatase Enzyme
Description:
This paper describes experiments aimed at developing conditions for suspension culturing of a producer clone of the recombinant lysosomal enzyme iduronate-2-sulfatase based on the CHO cell line.
As a result of sequential culturing in batch and fed-batch modes, the optimal nutrient medium composition was established, providing a 2.
5-fold increase in the iduronate-2-sulfatase specific activity.
Optimization of the feed formulation was carried out, which increased the yield of recombinant iduronate-2-sulfatase.
The ambr® 15 Cell Culture System of minibioreactors was used to optimize the cultivation process.
Chinese hamster ovary cells, CHO, iduronate-2-sulfatase, idursulfase, lysosomal enzyme, sulfatases, Аmbr Tap Biosystems, ambr® 15 Cell Culture System, mucopolysaccharidosis type II, formyl glycine generating enzyme, optimization of cell culturing conditions, fed-batch culture, batch culture.
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