Biochemical basis of resistance in green gram genotypes against pulse beetle, Callosobruchus maculatus (F.) under storage conditions

AbstractStudy on the biochemical basis of resistance in green gram genotypes against pulse beetle showed that highest seed damage was recorded in genotype Pant Mung-2 (69.25%) whereas, lowest seed damage was found in genotype ML-935 (46.50%). The maximum weight loss was recorded in genotype Pant Mung2 (43.00%) and minimum weight loss was recorded on ML-935 (11.67%). The lowest protein content was recorded in genotype ML-935 (11.50 mg/g) while, highest protein content was reported in genotype Samrat (27.70 mg/g) followed by Pant Mung-2 (26.90 mg/g). The genotypes Samrat (40.36 mg/g) had the highest starch content followed by Pant Mung-2 (39.00 mg/g) and genotype ML-935 (26.53 mg/g) had the lowest starch content. The genotype Samrat (8.66 mg/g) received highest sugar content followed by Pant Mung2 (7.90 mg/g) and genotype ML-935 (2.96 mg/g) received the lowest content of total soluble sugar. The genotype ML-935 (17.03 TIU/mg protein) showed highest trypsin inhibitor activity followed by ML1059 (14.50 TIU/ mg protein). The genotype Samrat (11.37 TIU/ mg protein) depicted lowest trypsin inhibitor activity followed by genotype Pant Mung-2 (11.90 TIU/ mg protein). The genotype ML-935 (29.06%) had the highest protease inhibitor activity followed by genotype ML1059 (26.50%). The genotype IPM-2-3 (17.16%) followed by genotype Ganga-8 (17.50%) showed protease inhibitor activity. A positive significant correlation was observed between per cent seed damage, per cent weight loss and biochemical parameters like protein content (r = 0.892** and r = 0.881**, respectively), starch content (r = 0.924** and r = 0.895**, respectively) and total soluble sugar (TSS) content (r = 0.886** and r = 0.855**, respectively), whereas parameters like trypsin inhibitor activity (r = -0.891** and r = -0.871****, respectively) and protease inhibitor activity (r = -0.937** and r = -0.921****, respectively) were negatively correlated.