Increased Numbers of Circulating Th22 and Th17 Cells in Children with Kawasaki Disease

Abstract Background T-helper (Th) 17 and Th22 cells are critical for the pathogenic process of Kawasaki Disease (KD). Methods A total of 43 children with freshly diagnosed KD and 20 healthy controls (HC) were quantified for the numbers of Th17, Th22 and Th1 cells by flow cytometry. The concentrations of serum IL-17, IL-22, IL-6, IFN-γ and TNF-α were examined by ELISA. Results Compared to those in the HC, significantly increased numbers of Th17 and Th22 cells, but not Th1 cells, and higher levels of serum IL-17 and IL-22, but not IFN-γ, were found in KD patients. Stratification analysis indicated the numbers of both Th17 and Th22 cells and the concentrations of serum IL-17 and IL-22 in KD patients with coronary artery lesions (CAL) were significantly greater than that in those with noncoronary artery lesions (NCAL). Treatment with the intravenous immunoglobulin (IVIG) therapy significantly decreased numbers of Th22 and Th17 cells as well as the serum concentrations of IL-22 and IL-17 in KD patients. The concentrations of serum IL-22 and IL-17 were correlated positively with C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) values as well as N-terminal pro-brain natriuretic peptide (NT-proBNP) in those patients respectively. Conclusions Our study provided direct evidence that Th22 and Th17 cells might contribute to the pathogenesis of KD.