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Inhibition of Isorhamnetin on β-Catenin/Tcf Signaling and β-Catenin-Activated Melanogenesis
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Activated β-catenin/T-cell factor (Tcf) signaling plays various roles in human cell mechanisms. We identified the inhibitory effect of isorhamnetin against β-catenin/Tcf signaling in β-catenin activated cells. Isorhamnetin inhibited the transcriptional activity of β-catenin/Tcf in HEK293 cells transiently transfected with a constitutively active mutant β-catenin gene. Also, isorhamnetin inhibited theβ-catenin-activated melanogenesis in B16F1 cells. Regarding the inhibitory mechanism, the shift assay showed that the binding of Tcf complexes with its specific DNA-binding sites was suppressed byisorhamnetin. Western blot analysis showed a decreased level of β-catenin in nucleus caused by isorhamnetin. These results suggest that the isorhamnetin acts as a negative regulator of β-catenin/Tcf signaling and its inhibitory mechanism is related to the decreased binding of β-catenin/Tcf complexes to consensus DNA.
Title: Inhibition of Isorhamnetin on β-Catenin/Tcf Signaling and β-Catenin-Activated Melanogenesis
Description:
Activated β-catenin/T-cell factor (Tcf) signaling plays various roles in human cell mechanisms.
We identified the inhibitory effect of isorhamnetin against β-catenin/Tcf signaling in β-catenin activated cells.
Isorhamnetin inhibited the transcriptional activity of β-catenin/Tcf in HEK293 cells transiently transfected with a constitutively active mutant β-catenin gene.
Also, isorhamnetin inhibited theβ-catenin-activated melanogenesis in B16F1 cells.
Regarding the inhibitory mechanism, the shift assay showed that the binding of Tcf complexes with its specific DNA-binding sites was suppressed byisorhamnetin.
Western blot analysis showed a decreased level of β-catenin in nucleus caused by isorhamnetin.
These results suggest that the isorhamnetin acts as a negative regulator of β-catenin/Tcf signaling and its inhibitory mechanism is related to the decreased binding of β-catenin/Tcf complexes to consensus DNA.
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