e0163 The association of furin gene coding polymorphism with essential hypertension in kazakan of Xinjiang

Introduction The proprotein processing enzyme furin is involved in many blood pressure-regulating factors. In the Golgi, furin appears to activate ENaC. Thus the gene of furin (FUR) may be a candidate gene of hypertension. We investigate the relationship between Furin gene polymorphism and hypertension in Xinjiang Kazakans. Methods 924 Kazakans was selected randomly from the pasturing area aged in Xinjiang (422 hypertensives, 502 normotensive controls). We sequenced the coding regions of FUR in 94 hypertensive individuals to identify genetic variations of FUR. Genotyping by the TaqMan-PCR method was performed for common SNPs. The possible relationship between the polymorphism and hypertension of Kazakan was analysed. Results 1. Direct sequencing from 94 hypertension subjects identified 6 SNPs in the Furin gene promoter. 2. The genotypes and allele frequencies of −229C→T, 12690G→C polymorphisms are not significant between these two groups. 3. There was no significance of blood pressure among the three genotypes of −229C→T, 12690G→C polymorphism. In multiple logistic analyses, the genotypes of the −229C>T were excluded as independent variables. None of haplotypes composed of −229C→T and 12690G→C was significantly different in EH and controls. Conclusion The Furin gene −229C→T, 12690G→C polymorphism might not be associated with essential hypertension in Kazakans.