LLPSWise - fast and accurate prediction of LLPS constituents

Abstract The recent discovery of different types of biological liquid-liquid phase separation (LLPS) systems presents enormous opportunities to uncover underlying biological mechanisms from a single-molecule level to mesoscopic scales and beyond. While progress has been made on the front of computational prediction of LLPS propensity of proteins, the constituent identification of LLPS systems continues to be a pertinent issue that has yet to be addressed. Compiled evidence indicates that the biological mechanism of LLPS involves the employment of different biomolecules into their liquid-liquid separation phase. Since constituent identification relies on experimental approaches, the process is arduous and inefficient. Here, we propose a sequence based LLPS propensity prediction method and scan the human proteome and biochemical pathways to establish a systematic biological view of LLPS. Additionally, we present a fast, accurate method for identifying the constituents of LLPS systems. The source code for our method is available via https://github.com/promethiume/LLPSWise