Differential block of nicotinic synapses on B versus C neurones in sympathetic ganglia of frog by α‐conotoxins MII and ImI

The effects of two new acetylcholine receptor antagonists, α‐conotoxin MII and α‐conotoxin ImI, on nicotinic synaptic transmission in the 10th paravertebral sympathetic ganglion of the leopard frog (Rana pipiens) were examined. The preganglionic nerve was electrically stimulated (at low frequency, 1 min−1, to avoid use‐dependent changes) while compound action potentials of B and C neurones were monitored from the postganglionic nerve. α‐Conotoxins MII and ImI, at low micromolar concentrations, reversibly blocked both B and C waves. α‐Conotoxin MII blocked the C wave more effectively than the B wave, whereas the potency of α‐conotoxin ImI was opposite that of MII. The observation that nicotinic antagonists can differentially block synaptic transmission of B versus C neurones with opposite selectivities strongly suggests that these neurones possess distinct nicotinic receptors. In addition to fast and slow B waves described by others, C waves with two temporally distinguishable components were present in our recordings. Each α‐conotoxin affected fast and slow B waves similarly. Likewise, toxins did not discriminate between the two components of C waves. This suggests that all neurones within each major class (B or C) may have the same nicotinic receptors. Synthetic forms of α‐conotoxins MII and ImI were used in the present study. Their ease of synthesis and their specificities should make these toxins useful probes to investigate the various subtypes of neuronal nicotinic acetylcholine receptors. British Journal of Pharmacology (1997) 120, 995–1000; doi:10.1038/sj.bjp.0700993