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Nova-ST Chip Preparation Protocol v1
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Nova-ST is a an open-source, high-resolution sequencing based spatial transcriptomics workflow. This method gives comparable resolution to BGI Stereoseq, SeqScope & PIXEL seq. Nova-ST is derived from dense nano-patterned randomly barcoded Illumina NovaSeq 6000 S4 sequencing flow cells. More details in the Nova-ST pre-print (https://www.biorxiv.org/content/10.1101/2024.02.22.581576v1). Nova-ST enables customized, low cost, flexible, and high-resolution spatial profiling of broad range of tissue section sizes (upto 10mm x 8 mm). In this protocol, we provide detailed step-by-step resource for implementing the Nova-ST spatial transcriptomics workflow in you lab. Bioinformatics and data analysis workflows are detailed in: https://github.com/aertslab/Nova-ST. For any protocol related or data analysis clarifications, you can reach out to us via nova.st.aertslab@gmail.com.
Title: Nova-ST Chip Preparation Protocol v1
Description:
Nova-ST is a an open-source, high-resolution sequencing based spatial transcriptomics workflow.
This method gives comparable resolution to BGI Stereoseq, SeqScope & PIXEL seq.
Nova-ST is derived from dense nano-patterned randomly barcoded Illumina NovaSeq 6000 S4 sequencing flow cells.
More details in the Nova-ST pre-print (https://www.
biorxiv.
org/content/10.
1101/2024.
02.
22.
581576v1).
Nova-ST enables customized, low cost, flexible, and high-resolution spatial profiling of broad range of tissue section sizes (upto 10mm x 8 mm).
In this protocol, we provide detailed step-by-step resource for implementing the Nova-ST spatial transcriptomics workflow in you lab.
Bioinformatics and data analysis workflows are detailed in: https://github.
com/aertslab/Nova-ST.
For any protocol related or data analysis clarifications, you can reach out to us via nova.
st.
aertslab@gmail.
com.
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