Efek Daun Picung (Pangium edule Rewind.) sebagai Pengawet Alami terhadap Kadar Protein Total Ikan Nila (Oreochromis sp) Segar

Tilapia (Oreochromis sp) is one group of fish that has high economic value. Tilapia contains protein that is easy to lysis, quickly undergoes a process of decay (perishable food). Alternatively the preventing process of preserving fish with natural ingredients is Picung leaves. The aim of study was to compare the levels of protein of fresh Tilapia before preserving with Tilapia preserved with Picung leaves (Pangium edule Rewins.) at concentrations of 5 %. This research method uses experiment with the purposive sampling with complete randomized analysis, using temperature and preservation factors with each treatment, namely 6 replications. Temperature factor that is using room temperature 28o C and temperature 18o C ( in refrigerator) and the preservation time factor is 0 hours, 12 hours, 24 hours, 36 hours, and 48 hours. Protein determination used spectrophotometric Kjedhal modification methods. The results of the average protein content of fresh Tilapia before being preserved are 19.93 %, the average protein of Tilapia is preserved at 28oC which is 13.59% and the average protein of Tilapia is preserved at 18oC which is 15.07%. The average protein of Tilapia was preserved for 12 hours, 24 hours, 36 hours and 48 hours, namely 13.22%, 13.88%, 12.78% and 11,83%. The research data was analysed by ANOVA test with result Fs Faktor A> F0.05 (1,50), meaning that there is a real effect caused by factor A (temperature factor). Fs Faktor B > F0.05 (4,50),meaning that there is real effect caused factor B (preservation time).Keywords: Tilapia, Protein, Picung Leaves and Natural PreservativeABSTRAKIkan nila (Oreochromis niloticus) adalah salah satu kelompok ikan yang mempunyai nilai ekonomis tinggi. Ikan Nila mengandung protein yang mudah lisis cepat mengalami proses pembusukan (perishable food). Alternatif mencegah proses tersebut dilakukan pengawetan ikan dengan bahan alami seperti daun picung. Tujuan penelitian  yaitu membandingkan kadar protein ikan Nila segar sebelum diawetkan dan setelah diawetkan dengan daun picung (Pangium edule Rewins.) pada konsentrasi 5%. Metoda penelitian ini menggunakan percobaan dengan purposive sampling. Rancangan acak lengkap, mengunakan faktor suhu dan waktu pengawetan dengan masing-masing perlakuan yaitu 6 ulangan . Faktor suhu yaitu menggunakan suhu ruang 28o C dan suhu 18o C (dalam kulkas) dan faktor waktu pengawetan yaitu 0 jam, 12 jam, 24 jam, 36 jam dan 48 jam. Penentuan protein mengunakan metode modifikasi kjedhal spektofotometri.  Hasil rerata kadar protein ikan Nila segar sebelum diawetkan yaitu 19.93%, rerata ikan Nila yang diawetkan  pada suhu 28o C yaitu 13.59 % dan rerata kadar protein ikan Nila yang diawetkan pada suhu 18o C yaitu 15.07%. Rerata protein ikan Nila yang diawetkan selama 12 jam, 24 jam, 36 jam dan 48 berurutan yaitu 13.22%, 13.88%, 12.78% dan 11.83%. Data penelitian ini dianalisa dengan uji Anova dengan hasil  Fs Faktor A > F0.05 (1,50), artinya terdapat efek yang nyata ditimbulkan oleh faktor A (faktor suhu). Nilai Fs Faktor B  > F0.05 (4,50) , artinya terdapat efek yang nyata ditimbulkan oleh faktor B( waktu pengawetan).Kata Kunci: Ikan Nila, Protein, Daun Picung, Pengawet Alami