A validated RP-HPLC method for quantitative determination of sodium taurocholate in bovine bile extract

Sodium taurocholate, a major component of bile salts, plays an important role in pharmaceutical and biochemical applications. This study aimed to develop and validate a high-performance liquid chromatography (HPLC) method for accurately determining sodium taurocholate in bovine bile extracts. Chromatographic separation was carried out using a Shodex C18-4E column (250 mm × 4.6 mm, 5 μm), with a mobile phase composed of methanol and 25 mM phosphate buffer solution, pH 5.45, at a flow rate of 0.7 mL/min. The analytes were detected with a UV detector set at 210 nm. The method was validated for selectivity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ). The calibration curve showed linearity over the range of 10–100 µg/mL, with a correlation coefficient ( r ) of 0.9997. Intra-day and inter-day precision ranged from 0.60% to 1.28%. Spike recovery of sodium taurocholate ranged from 100.01% to 100.93%. The limit of detection and limit of quantification for sodium taurocholate were 2.83 µg/mL and 8.57 µg/mL, respectively. The validated method ensures reliable and reproducible quantification, making it suitable for quality control applications.