Hyperthermia inhibited cellular function and induced immunogenic cell death of renal cell carcinoma

Abstract Background: As a novel strategy, hyperthermia has been widely applied to cancer treatment recent years for its multiple anti-tumor effects. Especially, the potential influences of hyperthermia on tumor immune microenvironment may contribute to improve the efficacy of immunotherapy. However, the role of hyperthermia on renal cell carcinoma (RCC) is not well understood until now. Methods: In present study, we primarily evaluated the effects of hyperthermia on cellular function via cellular proliferation, migration assay, invasion and apoptosis assays. In addition, the influence of hyperthermia on immunogenicity of RCC cells were analyzed using flow cytometry analysis, enzyme-linked immunosorbent assays and IF staining. Results: our results showed that hyperthermia could significantly inhibit the proliferation, migration and invasion of RCC cells, and promote cell apoptosis. In addition, hyperthermia was verified to improve the immunogenicity of RCC cells via inducing immunogenic cell death. Conclusion: Our data demonstrated that hyperthermia was expected to be a promising therapeutic strategy for RCC.