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In vivo CRISPR-Cas9 expression in Candida glabrata , Candida bracarensis and Candida nivariensis : a versatile tool to study chromosomal break repair.
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The CRISPR-Cas9 system is extremely useful for genome editing in many
species, including the model yeast
Saccharomyces cerevisiae
, and
other yeast species. We have previously reported the use of an inducible
CRISPR-Cas9 system in
Candida glabrata
, which allows genome
editing but also the study of Double Strand Break (DSB) repair. We
report, in this study, a comparable system for
C. glabrata
,
relying on a new plasmid, which is more stable than the previous one. We
also report the use of this plasmid to induce DSBs in two additional
human pathogens,
Candida bracarensis
and
Candida
nivariensis.
We examine lethality induced by an
in vivo
DSB in
the three species and describe the different types of Non-Homologous
End-Joining (NHEJ) events detected in these three pathogens.
Title: In vivo CRISPR-Cas9 expression in Candida glabrata , Candida bracarensis and Candida nivariensis : a versatile tool to study chromosomal break repair.
Description:
The CRISPR-Cas9 system is extremely useful for genome editing in many
species, including the model yeast
Saccharomyces cerevisiae
, and
other yeast species.
We have previously reported the use of an inducible
CRISPR-Cas9 system in
Candida glabrata
, which allows genome
editing but also the study of Double Strand Break (DSB) repair.
We
report, in this study, a comparable system for
C.
glabrata
,
relying on a new plasmid, which is more stable than the previous one.
We
also report the use of this plasmid to induce DSBs in two additional
human pathogens,
Candida bracarensis
and
Candida
nivariensis.
We examine lethality induced by an
in vivo
DSB in
the three species and describe the different types of Non-Homologous
End-Joining (NHEJ) events detected in these three pathogens.
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