Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Biological Activities of Molecules Derived from Olea europaea L. Tested In Vitro
View through CrossRef
Background: Extra virgin olive oil is a typical food of the Mediterranean area, obtained by pressing Olea europaea L. fruits. Its polyphenols have been studied for their antioxidant function and protective action against cancer and chronic kidney disease. In this in vitro study, we tested titrated extracts from Olea europaea L. on a human embryonic kidney 293 (HEK-293E) cell line, regarding their pro-apoptotic and antiproliferative capacities, using “ IncuCyte® S3 Live-Cell Analysis System”. Materials and Methods: We selected Olea europaea L. active compounds like hydroxytyrosol (HT) and oleuropein (OLE). These extracts were tested at different concentrations and characterized by HPLC-DAD-MS for the content in secondary active metabolites. The real-time observation of cell behavior was performed by IncuCyte, which can quantitatively analyze the cell proliferation and death. Results: This study showed that all the tested extracts can significantly inhibit cellular growth at 50 µM but the reduced proliferation is not related to an increase in cellular apoptosis. Instead, the same analysis performed by using extracts at 100 µM reveals that they can inhibit cellular growth, further inducing cellular apoptosis. Conclusions: The results on the HEK-293E cells confirmed the antiproliferative and proapoptotic actions of active compounds from an Olea europaea L. matrix in this cell line.
Title: Biological Activities of Molecules Derived from Olea europaea L. Tested In Vitro
Description:
Background: Extra virgin olive oil is a typical food of the Mediterranean area, obtained by pressing Olea europaea L.
fruits.
Its polyphenols have been studied for their antioxidant function and protective action against cancer and chronic kidney disease.
In this in vitro study, we tested titrated extracts from Olea europaea L.
on a human embryonic kidney 293 (HEK-293E) cell line, regarding their pro-apoptotic and antiproliferative capacities, using “ IncuCyte® S3 Live-Cell Analysis System”.
Materials and Methods: We selected Olea europaea L.
active compounds like hydroxytyrosol (HT) and oleuropein (OLE).
These extracts were tested at different concentrations and characterized by HPLC-DAD-MS for the content in secondary active metabolites.
The real-time observation of cell behavior was performed by IncuCyte, which can quantitatively analyze the cell proliferation and death.
Results: This study showed that all the tested extracts can significantly inhibit cellular growth at 50 µM but the reduced proliferation is not related to an increase in cellular apoptosis.
Instead, the same analysis performed by using extracts at 100 µM reveals that they can inhibit cellular growth, further inducing cellular apoptosis.
Conclusions: The results on the HEK-293E cells confirmed the antiproliferative and proapoptotic actions of active compounds from an Olea europaea L.
matrix in this cell line.
Related Results
Revealing suitable habitats for Juniperus procera and Olea europaea tree species in the remnant dry Afromontane forests of Ethiopia: Insights from ensemble species distribution modeling approach
Revealing suitable habitats for Juniperus procera and Olea europaea tree species in the remnant dry Afromontane forests of Ethiopia: Insights from ensemble species distribution modeling approach
AbstractHuman activities and climate change pose a significant threat to the dry Afromontane forests in Ethiopia, which are essential for millions of people both economically and e...
Facile Preparation of Iron Nanoparticles Mediated by the Extract of Olea europaea and Chitosan and Assessment of the Anti‐Osteoporosis Properties
Facile Preparation of Iron Nanoparticles Mediated by the Extract of Olea europaea and Chitosan and Assessment of the Anti‐Osteoporosis Properties
ABSTRACTOsteoporosis is characterized by a reduction in bone mineral density, compromised bone microarchitecture and mineralization, and diminished bone strength, which collectivel...
Antifungal activity of Olea europaea subsp. sylvestris tar against Fusari-um oxysporum f. sp. albedinis, the causal agent of Bayoud of the date palm in Southwest Algeria
Antifungal activity of Olea europaea subsp. sylvestris tar against Fusari-um oxysporum f. sp. albedinis, the causal agent of Bayoud of the date palm in Southwest Algeria
Bayoud, a vascular disease of date palm, caused by Fusarium oxysporum f. sp. albedinis (FOA) is the most damaging disease of date palm in Algeria, with no effective control strateg...
Influence of Some Plant Extracts on Antifungal Properties, Hardness, and Peel Bond Strength of Heat-Cured Denture Soft Liner
Influence of Some Plant Extracts on Antifungal Properties, Hardness, and Peel Bond Strength of Heat-Cured Denture Soft Liner
Background: Soft liners in dentures play a crucial role in enhancing patient comfort and preventing tissue irritation especially for patients with resorbed ridges. However, microbi...
Genoprotective and Antioxidant Effects of Olea ferruginea Leaf Extract Through Micronucleus and Antioxidant Assays
Genoprotective and Antioxidant Effects of Olea ferruginea Leaf Extract Through Micronucleus and Antioxidant Assays
Olea ferruginea has plenty of phenolic compounds with significant antioxidant potential, but its genoprotective effects against chemotherapeutic drug–induced DNA damage remains une...
Conservation Threats to Ethnomedicinal plants in Kore District, South Eastern, Ethiopia
Conservation Threats to Ethnomedicinal plants in Kore District, South Eastern, Ethiopia
The study aimed to investigate the threats to the biodiversity of ethnomedicinal plants and to find consensus information on conservation and management practices of ethnomedicinal...
Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves
Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves
One of the most prevalent environmental stresses that affects plants physically and physiologically is
the low temperature. Since low temperature adversely affects plant producti...
MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing v1
MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing v1
Human tissues comprise trillions of cells that populate a complex space of molecular phenotypes and functions and that vary in abundance by 4–9 orders of magnitude. Relying solely ...