The phytochemical investigation from n-hexane extract of the lichen Roccella montagnei

Introduction: R occella montagnei is widely distributed in subtropical regions. As the continuous study on the hexane extract of Roccella montagnei lichen, the isolation and structural determination of five compounds were addressed. Method: The crude extract was obtained from the dried lichen powder's extraction at room temperature. The n-hexane, n-hexane-ethyl acetate, and ethyl acetate extracts were obtained by the liquid-liquid partition method. The organic compounds were isolated from n-hexane extract by silica gel and Sephadex LH-20 column chromatography. Their chemical structures were identified by the NMR and HR-ESI-MS data analysis and the comparison of their NMR data with the published data. Results: Five compounds were isolated and chemically structural identified, consisting of 3b -hydroxy-7a-methoxystigmast-5-ene (1), sekikaic acid (2), lichenxanthone (3), (+)-6,8-dihydroxy-3-propyl-3,4-dihydroisocoumarin (4), and ar-turmerone (5). Conclusion: To the best of our knowledge, except 3 which was reported from this species for the first time, four isolated compounds left did not known to be present in Roccella genus before.