Effect of Solvents on Phytochemicals Content and Antioxidant Activity of Ganoderma lucidum

Introduction: The aim of this study was to assess the induction of solvents on the total phenol and flavonoid content and also the antioxidant activity of Ganoderma lucidum extracts. Materials & Methods: In this study, two concentrations (100% and 75%) of diethyl ether, ethanol, butanol, chloroform, and acetone were used as extractants of Ganoderma lucidum. Total phenol and flavonoid contents were measured by spectrophotometric methods and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Free radical scavenging assay was used for the investigation of antioxidant activity. Results & Discussion: Extractants significantly affected the % yield of extract, the quantity of phenol and flavonoids and antioxidant activity of Ganoderma lucidum mushroom. The highest extraction yield, around 38%, was achieved by 75% acetone, followed by 100% acetone (about 36%) and 75% chloroform (approximate 21%). Hydro-acetone extract exhibited the most significant antioxidative properties (EC50 value; 645.55 µg/mL) comprised of a higher total of phenol content. In conclusion, the total phenol content encouraged the antioxidative potential of Ganoderma lucidum mushroom. Conclusion: These findings indicate that the selective extraction of Ganoderma lucidum shows significant biological activities.