Examing the aniline degradation by Pseudomonas moraviensis AN-5

Aniline is a toxic aromatic compound causing environmental pollution, especially water problems, which harmfully affect to human and aquatic species. Seven bacterial strains were isolated from the sewerage of biological and chemical laboratories using aniline as a sole carbon and nitrogen source. Among them, Pseudomonas moraviensis AN-5 showed the highest efficiency. The aniline-degrading P. moraviensis AN-5 utilized aniline completely after 3 days at concentrations ≤ 4.0 mM. The addition of yeast extract and succinate stimulated the growth and aniline degradation of the strain. The investigation of aniline degraration pathway of P. moraviensis AN-5 showed that aniline was degraded by AN-5 via the ortho-clevage pathway.