Rab21

Abstract Rab21 was first isolated by a polymerase chain reaction (PCR)-based approach with degenerate oligonucleotides as a cDNA fragment containing a portion of the open reading frame between the first and second conserved regions involved in GTP binding (originally named Pcr-Rab12) (Chavrier et al. 1992). The full-length Rab21 cDNA was cloned by probing a MDCKII cell cDNA library with an oligonucleotide corresponding to the sequence FNDKHIT-TLQAS specific for Rab21 (Chavrier and Zerial, unpublished). The DNA sequence of canine Rab21 is shown in Fig. 1. Northern-blot analysis performed with the Rab21 cDNA probe reveals three major transcripts of approximately 1.6,2.5 and 4.0 kb expressed in baby hamster kidney (BHK) cells (Chavrier eta/. 1992).