A STUDY OF MICROBIAL CONTAMINATION IN PUNARNAVA MANDURA

Introduction: Herbal medicines are being used extensively in many countries to treat many diseases, as seen in the COVID-19 pandemic, so the global market for herbal medicines has grown suddenly. In India, under the Ayurveda system of medicine, apart from plants, animal and mineral products have also been used as medicine since long ago. Both herbal and animal products contain primarily organic components; therefore, like food in-gredients (biological ingredients), herbal medicine and animal products are at risk of microbial growth and con-tamination. Punarnava Mandura is a popular Ayurvedic medicine described in various classical and authoritative texts of Ayurveda. It is a perfect example of a medicine made from substances derived from plants, minerals, and animals. It contains Mandura Bhasma as a mineral component apart from other herbal ingredients, while cow urine (Gomutra) is of animal origin. It is widely used in infectious and non-infectious fever, cold, indigestion, and other diseases of the Gastrointestinal tract. Microbial contamination of medicines is defined as the unintend-ed habitation of pathogenic microbes that cause their degradation. In pharmaceutics, various factors may be a source of microbes, including the ingredients of drugs, manpower, machines, and the environment of the phar-macy. Microbial contamination is a pertinent issue and essential in quality control steps in pharmaceutical man-ufacturing and food processing. Microbial contamination of medicines and food products may also affect human health. Objective: The primary aim and purpose of the present study was to determine the microbial contamina-tion of an Ayurvedic Herbo-mineral drug sample of Punarnava Mandura. Material and methods: The drug sam-ple (Self-made) was made in the institute pharmacy. Another drug sample (market sample) of Punarnava Man-dura of a pharmacy was also kept as a control to compare the results. Quantitative and qualitative assessment of microbial loads in Punarnava Mandura was assessed. The following strains of microbes: E. Coli, Salmonella spp., Staphylococcus aureus, Fungus, and Total viable aerobic bacterial count were evaluated. Results: Both the samples of Punarnava Mandura have shown an insignificant load of selected microbes.